產(chǎn)品介紹 品名 Monoclonal Antibody to Human S Protein (Vitronectin) 貨號(hào) A237 規(guī)格 100 μL 同種型 IgG1k 蛋白質(zhì)濃度 > 1.0 mg/mL 純度 ≥ 95% by SDS PAGE 緩沖液 Borate Buffered Saline (pH 8.4 ± 0.2) 物種交叉反應(yīng) None tested. 使用說明 For Research Use Only.Not for use in Diagnostic Procedures. 產(chǎn)品描述 A murine monoclonal antibody to human Vitronectin. This monoclonal antibody was raised against purified human S Protein (Vitronectin). Quidel's S Protein monoclonal antibody has been used in a variety of experimental techniques including EIA and Western Blot. 背景 In contract to membrane-formed C5b-9 complexes, C5b-9 complexes formed in the fluid phase contain an additional protein, the S protein. Subsequently, it was found that isolated S protein inhibited C5b-9 attachment. S protein functions like a “membrane analogue” in that it binds to the binding site generated on assembly of C5b-9 and is required for insertion of the complex into the membrane. As a result, SC5b-7, SC5b-8 or SC5b-9 complexes are formed which have lost their membrane-binding capacity. When the S protein was cloned and sequenced, it was found to be identical to Vitronectin. Within the complement cascade, Vitronectin not only inhibits attachment of C5b-9 to target cells, but also prevents C9 polymerization. The biological function of Vitronectin is to minimize complement-mediated attack of cells in the vicinity of an ongoing complement attack. This may be relevant, not only during the generation of bactericidal activity in the case of infection, but also in autoimmune disease, immune complex disease, or inflammatory lesions, where complement is readily activated by dead or dying cells, exposed basement membranes or matrix proteins. S protein is present in human plasma at an approximate concentration of 150 μg/mL. 特異性 The specificity of the monoclonal antibody was established via ELISA and RIA. This antibody was shown to bind S protein (Vitronectin) immobilized in microtiter wells and immunoprecipitates highly purified, radiolabeled S protein (Vitronectin). It binds to the SC5b-9 complex 應(yīng)用 參考資料 1、Verhuis, R., van der Valk, P. Complement activation in amyloid plaques in Alzheimer's Disease does not proceed further than C3, Virchos Arch 426:603-610, 1995. 2、Rosoklija, GB. et al. Local activation of the complement system in endoneurial microvessels of diabetic neuropathy. Acta Neuropathol 99(1):55-62 (2000)
產(chǎn)品介紹
Monoclonal Antibody to Human S Protein (Vitronectin)
A237
100 μL
IgG1k
> 1.0 mg/mL
≥ 95% by SDS PAGE
Borate Buffered Saline (pH 8.4 ± 0.2)
None tested.
For Research Use Only.Not for use in Diagnostic Procedures.
A murine monoclonal antibody to human Vitronectin.
This monoclonal antibody was raised against purified human S Protein (Vitronectin).
Quidel's S Protein monoclonal antibody has been used in a variety of experimental techniques including EIA and Western Blot.
In contract to membrane-formed C5b-9 complexes, C5b-9 complexes formed in the fluid phase contain an additional protein, the S protein. Subsequently, it was found that isolated S protein inhibited C5b-9 attachment. S protein functions like a “membrane analogue” in that it binds to the binding site generated on assembly of C5b-9 and is required for insertion of the complex into the membrane. As a result, SC5b-7, SC5b-8 or SC5b-9 complexes are formed which have lost their membrane-binding capacity. When the S protein was cloned and sequenced, it was found to be identical to Vitronectin.
Within the complement cascade, Vitronectin not only inhibits attachment of C5b-9 to target cells, but also prevents C9 polymerization. The biological function of Vitronectin is to minimize complement-mediated attack of cells in the vicinity of an ongoing complement attack. This may be relevant, not only during the generation of bactericidal activity in the case of infection, but also in autoimmune disease, immune complex disease, or inflammatory lesions, where complement is readily activated by dead or dying cells, exposed basement membranes or matrix proteins. S protein is present in human plasma at an approximate concentration of 150 μg/mL.
The specificity of the monoclonal antibody was established via ELISA and RIA. This antibody was shown to bind S protein (Vitronectin) immobilized in microtiter wells and immunoprecipitates highly purified, radiolabeled S protein (Vitronectin). It binds to the SC5b-9 complex
1、Verhuis, R., van der Valk, P. Complement activation in amyloid plaques in Alzheimer's Disease does not proceed further than C3, Virchos Arch 426:603-610, 1995.
2、Rosoklija, GB. et al. Local activation of the complement system in endoneurial microvessels of diabetic neuropathy. Acta Neuropathol 99(1):55-62 (2000)
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