Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene. With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support). This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
General properties
Appearance:
yellowish foam
Molecular weight:
955.04
Molecular formula:
C57H55N4O8P
Solubility:
good in dichloromethane and acetonitrile
Quality control:
NMR 1H (95%) and 13C, TLC, HPLC
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
260; 282; 365; 392
Extinction coefficient at excitation maximum, Lmol-1cm-1:
12600; 21900; 16000; 14200
Emission maximum, nm:
460
Fluorescence quantum yield:
~0.1
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