High loading controlled pore glass solid support for the synthesis of oligonucleotides with 3'-alkyne group. With this solid support, synthesis of oligos of up to 50 bases is possible. Terminal alkyne group can be modified by copper catalyzed Click chemistry (see our recommended protocol for oligonucleotide modification). This solid support is compatible with standard oligonucleotide deblocking conditions. No special deblocking is required.
相關(guān)產(chǎn)品:
Alkyne Amidite, hydroxyprolinol - alkyne phosphoramidite with DMT group for cartridge and HPLC purification Alkyne Phosphoramidite, 5'-terminal - solid alkyne phosphoramidite for 5'-terminal modification which is easy to handle and dispense
General properties
Appearance:
off-white beads
Quality control:
NMR 1H and HPLC (95%) of bound reagent, loading measurement
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
CPG details
Pore size, ?:
500
Typical loading, umol/g:
70?90
Coupling conditions:
standard coupling, identical to normal nucleobases
Cleavage conditions:
ammonia, 2 h at room temperature
Deprotection conditions:
identical to protected nucleobases
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