SYBR Green I是一種敏感的dsDNA結(jié)合染料，可以用于日常瓊脂糖和聚丙烯酰胺凝膠的DNA檢測。 不同于Ethidium，SYBR Green I對雙鏈DNA有高度選擇性，更低的毒性，更高的靈敏度。
溴化乙錠（Ethidium Bromide）和SYBR Green I的對比
Feature
Ethidium Bromide
SYBR Green I
Fluorescence
Red (615 nm)
Green (524 nm)
Excitation maximum
302 nm
454 nm
Excitation light source
UV only
Blue light or UV
Sensitivity
2 ng / band(dsDNA) 100 ng / band (RNA)
0.08 ng / band (dsDNA) 1-2 ng / band (oligonucleotides)
Health hazard
High
Low
推薦手冊： SYBR Green I DNA凝膠染色 Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
Appearance:
orange solution
Quality control:
NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
454
Extinction coefficient at excitation maximum, Lmol-1cm-1:
73000
Emission maximum, nm:
524
Fluorescence quantum yield:
0.8
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