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上海拜力生物科技有限公司
Shanghai Bioleaf Biotech Co.,Ltd
電話 021-33779008
銷售**：4009687988
郵箱 info@bioleaf.com
網(wǎng)址 http://www.bioleaf.com/
地址 上海市閔行區(qū)新源路1356弄1-7號C801
郵編 201108
微信公眾號：拜力生物
| 訂購貨號 | 產(chǎn)品名稱及規(guī)格 | 價格（￥） |
| 20010 | dsGreen Gel Staining Solution, 10000×, 1 mL | 2340.00 |
SYBR Green I是一種敏感的dsDNA結(jié)合染料，可以用于日常瓊脂糖和聚丙烯酰胺凝膠的DNA檢測。
不同于Ethidium，SYBR Green I對雙鏈DNA有高度選擇性，更低的毒性，更高的靈敏度。
溴化乙錠（Ethidium Bromide）和SYBR Green I的對比
|
Feature |
Ethidium Bromide |
SYBR Green I |
|
Fluorescence |
Red (615 nm) |
Green (524 nm) |
|
Excitation maximum |
302 nm |
454 nm |
|
Excitation light source |
UV only |
Blue light or UV |
|
Sensitivity |
2 ng / band(dsDNA) |
0.08 ng / band (dsDNA) |
|
Health hazard |
High |
Low |
推薦手冊：
SYBR Green I DNA凝膠染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
|
Appearance: |
orange solution |
|
Quality control: |
NMR 1H (95%) and 13C, TLC, functional testing |
|
Storage conditions: |
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
|
MSDS: |
Download |
Spectral properties
|
Excitation maximum, nm: |
454 |
|
Extinction coefficient at excitation maximum, Lmol-1cm-1: |
73000 |
|
Emission maximum, nm: |
524 |
|
Fluorescence quantum yield: |
0.8 |